abstract：:The action of sialyltransferases (STs) on cell surface glycoconjugates is a key process in shaping cell phenotype in a variety of cells mostly involved in migratory and adhesive pathways. The factors determining cell-specific pattern of glycosylation are so far poorly understood. Most STs are resident proteins of the ...

journal_title：Biochimie

authors： Donadio S,Dubois C,Fichant G,Roybon L,Guillemot JC,Breton C,Ronin C

更新日期：2003-03-01 00:00:00

abstract：:The methylotrophic yeast Pichia pastoris is often used as an organism for the heterologous expression of proteins and has been used already for production of a number of glycosyltransferases involved in the biosynthesis of N- and O-linked oligosaccharides. In our recent studies, we have examined the expression in P. p...

journal_title：Biochimie

authors： Bencúrová M,Rendić D,Fabini G,Kopecky EM,Altmann F,Wilson IB

更新日期：2003-03-01 00:00:00

abstract：:Bikunin (Bk) is a Künitz-type serine proteinase inhibitor, which occurs in human plasma, mainly as covalent complexes with one or two of the three peptide heavy chains. The leading member of this glycoprotein family is inter-alpha-inhibitor (I alpha I), which consists of two heavy chains (H1 and H2) linked to Bk. Bk c...

journal_title：Biochimie

authors： Capon C,Mizon C,Lemoine J,Rodié-Talbère P,Mizon J

更新日期：2003-01-01 00:00:00

abstract：:Polysialylated neural cell adhesion molecule (NCAM) was immunoaffinity-purified from the brains of newborn calves. A degree of polymerization of up to 40 was chromatographically determined for released polysialic acid (PSA) chains. For characterization of N-glycan structures and attachment sites, PSA-NCAM was digested...

journal_title：Biochimie

authors： Wuhrer M,Geyer H,von der Ohe M,Gerardy-Schahn R,Schachner M,Geyer R

更新日期：2003-01-01 00:00:00

abstract：:The nuclear metabolism of poly(ADP-ribose) is mainly regulated by poly(ADP-ribose) polymerase-1 (PARP-1) and by poly(ADP-ribose) glycohydrolase (PARG). A PARP-like enzyme, V-PARP, and a PARG isoform are present in the extra-nuclear compartment of mammalian cells, even if poly(ADP-ribose) has never been detected therei...

journal_title：Biochimie

authors： Rossi L,Denegri M,Torti M,Poirier GG,Ivana Scovassi A

更新日期：2002-12-01 00:00:00

abstract：:The presence of marker genes encoding antibiotic or herbicide resistances in genetically modified plants poses a number of problems. Various techniques are under development for the removal of unwanted marker genes, while leaving required transgenes in place. The aim of this brief review is to describe the principal m...

journal_title：Biochimie

authors： Scutt CP,Zubko E,Meyer P

更新日期：2002-11-01 00:00:00

abstract：:The intervention of pyruvate in glucose metabolism was investigated during hypoxic stress in tumour cell cultures having respiratory capacities under normoxic conditions. Results obtained with nuclear magnetic resonance (NMR) spectroscopy showed that, under normoxic conditions, rat glioma C6 and human hepatoma Hep G2 ...

journal_title：Biochimie

authors： Perrin A,Roudier E,Duborjal H,Bachelet C,Riva-Lavieille C,Leverve X,Massarelli R

更新日期：2002-10-01 00:00:00

abstract：:Coenzymes are small organic molecules that supply a varied set of reactive groups to protein enzymes, thereby diversifying catalysis beyond the chemistries of amino acid sidechains. As RNA structures begin with a more limited chemical diversity than proteins, it seems likely that RNA enzymes would also use functional ...

journal_title：Biochimie

authors： Jadhav VR,Yarus M

更新日期：2002-09-01 00:00:00

abstract：:tmRNA (10Sa RNA, ssrA) acts to rescue stalled bacterial ribosomes while encoding a peptide tag added trans-translationally to the nascent peptide, targeting it for proteolysis. Ribosomal protein S1 is required for tmRNA binding to isolated and poly U-programmed ribosomes. Mobility assays on native gels indicate that t...

journal_title：Biochimie

authors： Bordeau V,Felden B

更新日期：2002-08-01 00:00:00

abstract：:The complete nucleotide sequence of a nerve growth factor precursor from Bothrops jararacussu snake (Bj-NGF) was determined by DNA sequencing of a clone from cDNA library prepared from the poly(A) + RNA of the venom gland of B. jararacussu. cDNA encoding Bj-NGF precursor contained 723 bp in length, which encoded a pre...

journal_title：Biochimie

authors： Kashima S,Soares AM,Roberto PG,Pereira JO,Astolfi-Filho S,Cintra AO,Fontes MR,Giglio JR,de Castro França S

更新日期：2002-07-01 00:00:00

abstract：:Ton-dependent colicins and microcins are actively taken up into sensitive cells at the expense of energy which is provided by the proton motive force of the cytoplasmic membrane. The Ton system consisting of the proteins TonB, ExbB and ExbD is required for colicin and microcin import. Colicins as well as the outer mem...

journal_title：Biochimie

authors： Braun V,Patzer SI,Hantke K

更新日期：2002-05-01 00:00:00

abstract：:The formation of integral membrane voltage-gated ion channels by the initially soluble C-terminal channel polypeptide (CP) of the pore-forming colicins is a fruitful area for studies on membrane protein import. The dependence of CP import on specific membrane parameters can be better understood using liposomes and pla...

journal_title：Biochimie

authors： Zakharov SD,Cramer WA

更新日期：2002-05-01 00:00:00

abstract：:The 11 kb las locus, present on the 50 kb plasmid pCIM1, specifies the production of the lantibiotic lactocin S in Lactobacillus sakei L45. The gene cluster is organized into two oppositely orientated operons, lasAMNTUVPJW (lasA-W) and lasXY, the former of which contains the biosynthetic, immunity and transport genes....

journal_title：Biochimie

authors： Rawlinson EL,Nes IF,Skaugen M

更新日期：2002-05-01 00:00:00

abstract：:Polynucleotide phosphorylase (PNPase, polyribonucleotide nucleotidyltransferase, EC 2.7.7.8) is a multifunctional protein, with a 3'-5' processive exoribonuclease, a Pi exchange, an RNA polymerase and an autoregulatory activity. The interaction between this enzyme and the mRNA target is crucial for its activities. In ...

journal_title：Biochimie

authors： Bermúdez-Cruz RM,García-Mena J,Montañez C

更新日期：2002-04-01 00:00:00

abstract：:Changes in the cytosolic Ca(2+) concentration ([Ca(2+)](c)) translate a variety of extracellular signals into widely diverse intracellular effects, ranging from secretion to movement, proliferation and also cell death. As regards the last one, it has long been known that large [Ca(2+)](c) increases lead cells to death...

journal_title：Biochimie

authors： Pinton P,Ferrari D,Rapizzi E,Di Virgilio F,Pozzan T,Rizzuto R

更新日期：2002-02-01 00:00:00

abstract：:TP53 is mutated in most types of human cancers and is one of the most popular genes in cancer research. The p53 protein is a sensor of multiple forms of genotoxic, oncogenic and non-genotoxic stress. It suppresses growth and controls survival of stressed cells, and as such, is the focal point of selection pressures in...

journal_title：Biochimie

authors： Guimaraes DP,Hainaut P

更新日期：2002-01-01 00:00:00

abstract：:Our understanding of plant meiosis is rapidly increasing thanks to the model Arabidopsis thaliana. Here we present the results of a screening for meiotic mutants carried out with a library containing 30,719 T-DNA insertion lines. An average of one mutant per 1000 lines was recovered. Several phenotypic classes could b...

journal_title：Biochimie

authors： Mercier R,Grelon M,Vezon D,Horlow C,Pelletier G

更新日期：2001-11-01 00:00:00

abstract：:Phosphoenolpyruvate carboxykinase (PEPCK) is the key enzyme in glyceroneogenesis, an important metabolic pathway that functions to restrain the release of non-esterified fatty acids (NEFAs) from adipocytes. The antidiabetic drugs known as thiazolidinediones (TZDs) are thought to achieve some of their benefits by lower...

journal_title：Biochimie

authors： Glorian M,Duplus E,Beale EG,Scott DK,Granner DK,Forest C

更新日期：2001-10-01 00:00:00

abstract：:Four full-length cDNAs encoding the precursors of four K(+)-toxin-like peptides (named BmKK(1), BmKK(2), BmKK(3) and BmmKK(4), respectively) were first isolated from a venom gland cDNA library of the Chinese scorpion Buthus martensii Karsch. The deduced precursors of BmKK(1), BmKK(2) and BmKK(3) are all made of 54 ami...

journal_title：Biochimie

authors： Zeng XC,Peng F,Luo F,Zhu SY,Liu H,Li WX

更新日期：2001-09-01 00:00:00

abstract：:The Golgi apparatus serves as the major site of glycosylation reactions. Nucleotide sugars which are substrates of the Golgi localized glycosyltransferases are synthesized in the cytoplasm (cell nucleus in case of CMP-sialic acid) and must be transported into the compartment lumen. This transport function is carried o...

journal_title：Biochimie

authors： Gerardy-Schahn R,Oelmann S,Bakker H

更新日期：2001-08-01 00:00:00

abstract：:Classic studies suggested that the common mammalian sialic acid N-glycolylneuraminic acid (Neu5Gc) is an oncofetal antigen in humans, being immunogenic in adult humans and yet apparently expressed in human fetuses and tumors. We and others have recently found that the human deficiency of Neu5Gc can be explained by an ...

journal_title：Biochimie

authors： Varki A

更新日期：2001-07-01 00:00:00

abstract：:Rhizopus oryzae lipase (ROL) was found to be a true lipase. This enzyme presents the interfacial activation phenomenon. The N-terminal amino acid sequence of ROL was compared to those of rhizopus lipases. Purified ROL possesses the same N-terminal sequence as the mature Rhizopus niveus lipase (RNL). This sequence was ...

journal_title：Biochimie

authors： Ben Salah A,Sayari A,Verger R,Gargouri Y

更新日期：2001-06-01 00:00:00

abstract：:Although believed to be widely distributed among the animal kingdom, transforming growth factor-beta (TGF-beta) superfamily members have mainly been characterized in vertebrate and in invertebrate model organisms such as Drosophila and C. elegans. To characterize such new factors in invertebrates, a PCR screen was per...

journal_title：Biochimie

authors： Lelong C,Mathieu M,Favrel P

更新日期：2001-05-01 00:00:00

abstract：:Oxidatively modified proteins are continuously produced in cells by reactive oxygen and nitrogen species generated as a consequence of aerobic metabolism. During periods of oxidative stress, protein oxidation is significantly increased and may become a threat to cell survival. In eucaryotic cells the proteasome has be...

journal_title：Biochimie

authors： Davies KJ

更新日期：2001-03-01 00:00:00

abstract：:The proteasome activators known as 11S REG or PA28 were discovered about 10 years ago. They are homo- or heteroheptameric rings that bind to the ends of 20S proteasomes and activate cleavage of peptides but not folded proteins. In this article, we focus on structural features of three homologous REG subunits (termed a...

journal_title：Biochimie

authors： Li J,Rechsteiner M

更新日期：2001-03-01 00:00:00

abstract：:We have analysed the complete sequence of the Escherichia coli K12 isolate MG1655 genome for chromatin-associated protein binding sites, and compared the predicted location of predicted sites with experimental expression data from 'DNA chip' experiments. Of the dozen proteins associated with chromatin in E. coli, only...

journal_title：Biochimie

authors： Ussery D,Larsen TS,Wilkes KT,Friis C,Worning P,Krogh A,Brunak S

更新日期：2001-02-01 00:00:00

abstract：:DNA replication in Escherichia coli is controlled at the initiation stage, possibly by regulation of the essential activity of DnaA protein. The cellular membrane has long been hypothesized to be involved in chromosomal replication. Accumulating evidence, both in vitro and in vivo, that supports the importance of memb...

journal_title：Biochimie

authors： Crooke E

更新日期：2001-01-01 00:00:00

abstract：:The mechanism responsible for creating the division site in the right place at the right time in bacteria is unknown. It has been attributed to the formation of proteolipid domains in the cytoplasmic membrane surrounding the nucleoids. We interpret the growing evidence for this hypothesis by invoking hyperstructures, ...

journal_title：Biochimie

authors： Norris V,Fishov I

更新日期：2001-01-01 00:00:00

abstract：:The Escherichia coli protein synthesis initiation factor IF2 is a member of the large family of G-proteins. Along with translational elongation factors EF-Tu and EF-G and translational release factor RF-3, IF2 belongs to the subgroup of G-proteins that are part of the prokaryotic translational apparatus. The roles of ...

journal_title：Biochimie

authors： Larigauderie G,Laalami S,Nyengaard NR,Grunberg-Manago M,Cenatiempo Y,Mortensen KK,Sperling-Petersen HU

更新日期：2000-12-01 00:00:00

abstract：:Esterases and lipases both hydrolyse ester bonds. Whereas the lipases display high activity towards the aggregated state of its substrate, the esterases typically show highest activity towards the soluble state of its substrate. We have compared the amino acid sequence, the 3D-structure as well as the pH-dependent ele...

journal_title：Biochimie

authors： Fojan P,Jonson PH,Petersen MT,Petersen SB

更新日期：2000-11-01 00:00:00

abstract：:There are five subtypes of muscarinic acetylcholine receptors (M(1) to M(5)) which control a large number of physiological processes, such as the function of heart and smooth muscles, glandular secretion, release of neurotransmitters, gene expression and cognitive functions as learning and memory. A selective ligand i...

journal_title：Biochimie

authors： Karlsson E,Jolkkonen M,Mulugeta E,Onali P,Adem A

更新日期：2000-09-01 00:00:00

abstract：:alpha-Latrotoxin, a protein toxin present in the venom of black widow spider, interacts with membrane receptors of neurons and other secretory cells to stimulate exocytosis. Two types of receptors have been identified and cloned. Our attention has been focused on the calcium independent receptor, a G-protein coupled r...

journal_title：Biochimie

authors： Pescatori M,Grasso A

更新日期：2000-09-01 00:00:00

abstract：:The yeast Saccharomyces cerevisiae strain LB332 bearing a mutation in the ERG20 gene encoding farnesyl diphosphate synthase (FPPS) synthesizes significantly longer dolichols than the wild type strain FL100 (14-31 and 14-19 isoprene units, respectively). The measurement of the short chain prenyl alcohols excreted into ...

journal_title：Biochimie

authors： Plochocka D,Karst F,Swiezewska E,Szkopińska A

更新日期：2000-08-01 00:00:00

abstract：:The synthesis of holocytochromes in plastids is a catalyzed process. Several proteins, including plastid CcsA, Ccs1, possibly CcdA and a thioredoxin, plus at least two additional Ccs factors, are required in sub-stoichiometric amounts for the conversion of apocytochromes f and c(6) to their respective holoforms. CcsA,...

journal_title：Biochimie

authors： Nakamoto SS,Hamel P,Merchant S

更新日期：2000-06-01 00:00:00

abstract：:Botulinum neurotoxins (BoNT, serotypes A-G) and tetanus neurotoxin (TeNT) are bacterial proteins that comprise a light chain (M(r) approximately 50) disulfide linked to a heavy chain (M(r) approximately 100). By inhibiting neurotransmitter release at distinct synapses, these toxins cause two severe neuroparalytic dise...

journal_title：Biochimie

authors： Humeau Y,Doussau F,Grant NJ,Poulain B

更新日期：2000-05-01 00:00:00

abstract：:Neuropeptides and hormones, in contrast to constitutive secretory proteins, are sorted to and stored in secretory granules and released upon a stimulus. During the last two decades, signals and mechanisms involved in their sorting to the regulated pathway of protein secretion have been addressed in numerous studies. T...

journal_title：Biochimie

authors： Glombik MM,Gerdes HH

更新日期：2000-04-01 00:00:00

abstract：:Gallium, a group IIIa metal salt, has been demonstrated to be an effective immunosuppressive agent. Gallium has also been shown to inhibit the production of inflammatory cytokines, such as IL-1beta, produced by macrophage-like cells in vitro. To further characterize the effects of gallium on the inflammatory process, ...

journal_title：Biochimie

authors： Panagakos FS,Kumar E,Venescar C,Guidon P

更新日期：2000-02-01 00:00:00

abstract：:Replication protein A (RPA) is a heterotrimeric single-stranded DNA binding protein whose role in DNA replication, recombination and repair has been mainly elucidated through in vitro biochemical studies utilizing the mammalian complex. However, the identification of homologs of all three subunits in Saccharomyces cer...

journal_title：Biochimie

authors： Smith J,Zou H,Rothstein R

更新日期：2000-01-01 00:00:00

abstract：:The antiviral activity of L-nucleoside analogs depends in part on the enantioselectivity of nucleoside kinases which catalyse their monophosphorylation. The substrate properties of human recombinant deoxycytidine kinase (dCK) and human recombinant deoxyguanosine kinase (dGK) with respect to L-adenosine and L-guanosine...

journal_title：Biochimie

authors： Gaubert G,Gosselin G,Boudou V,Imbach JL,Eriksson S,Maury G

更新日期：1999-11-01 00:00:00

abstract：:Mitochondrial function requires genes encoded in both mitochondrial and nuclear genomes. Tfam, the activator of mammalian mitochondrial transcription, is encoded in the nucleus and its expression has been shown in in vitro studies to be controlled by nuclear respiratory factors NRF-1 and NRF-2. In order to understand ...

journal_title：Biochimie

authors： Escrivá H,Rodríguez-Peña A,Vallejo CG

更新日期：1999-10-01 00:00:00